T5076
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 | Immunofluorescence Mouse cortical neurons labeled with Monoclonal Anti-β-Tubulin, Isotype III (Cat. No. T5076) (red) and anti-IL15 (green). From Diego Gomez Nicola, Functional and Systems Neurobiology, Cajal Institute (CSIC), Madrid, Spain. |
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T5076
Sigma
Monoclonal Anti-β-Tubulin Isotype III antibody produced in mouse
~2 mg/mL, clone SDL.3D10, purified immunoglobulin, buffered aqueous solution
Description
| Immunogen | peptide corresponding to the carboxy-terminal sequence of human β-tubulin isotype III, conjugated to BSA. |
| Specificity | By immunoblotting or immunofluorescence, the antibody does not bind tubulin in chicken brain preparation or chicken fribroblasts. |
| Physical form | Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | SDL.3D10 (monoclonal) |
| form | buffered aqueous solution |
| species reactivity | human, bovine, rat, mouse, boar |
| concentration | ~2 mg/mL |
| application(s) | dot blot: suitable |
| immunoblotting: 0.1-0.2 μg/mL using rat brain extract | |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| isotype | IgG2b |
| Gene Information | human ... TUBB3(10381) |
| rat ... Tubb3(246118) | |
| mouse ... Tubb3(22152) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
References
| reference | Diego Gomez-Nicola, Beatriz Valle-Argos, D. Wolfgang Pita-Thomas, Manuel Nieto-Sampedro, Interleukin 15 Expression in the CNS: Blockade of Its Activity Prevents Glial Activation After an Inflammatory Injury Glia 56, 494-505, (2008) Abstract |
